Modified mattress sutures vs running sutures in uterine closure: which is better?

Background: Conventional closure of uterus has been known to bear risk of scar dehiscence and scar rupture in subsequent pregnancies and thus, a study was conducted to compare the outcome of uterine closure with modified mattress manner and running manner and to establish a better method of uterine closure during caesarean section. Objective was to compare the conventional single layer running sutures and single layer modified mattress sutures for closure of uterus in caesarean section and find out which method is superior.Methods: This prospective interventional study was carried out in Dhiraj Hospital, a tertiary care hospital in Vadodara. 60 pregnant women in the study criteria were equally divided randomly into 2 groups. Uterine closure was done in single layered sutures, one by running sutures and other group by modified mattress sutures.Results: Uterine scar thickness on 8th day and 6 months post-operatively was significantly more in single layered suturing by modified mattress suture compared to running suture (p <0.05).Conclusions: Uterine closure by single layered modified mattress suture is better in comparison to conventional single layer running suture.

Screening and characterization of protease from bacillus sp.

Protease production by alkalophilic Bacillus sp were isolated from different regions of Bangalore and used to screen for the protease production by using casein and skim milk agar plate assay. The agro wastes are also used to screen and produce protease. The agro wastes are rice bran, paddy straw and pigeon pea waste, among these substrate rice bran showed maximum synthesis of protease. At 48 hr it showed maximum 0.13 IU protease production in rice bran medium was used for partial purification studies. Fermented medium were used for salt precipitation, dialysis. The dayalyste were used for studies on effect of Ph, temperature, inhibitors and metal ions. The optimum pH 10, temperature 650C were optimum and the inhibitors and metal ion studies, metalions like Mn2+ and Ca2+ were found to be potent enhancers. The complete loss of enzyme activity was found in presence of PMSF.